Inhibition of keratinocyte proliferation by extracts of Actinobacillus actinomycetemcomitans.
نویسنده
چکیده
A heat-labile sonic extract of a nonleukotoxic strain of Actinobacillus actinomycetemcomitans caused a dose-dependent inhibition of proliferation of human keratinocytes. Exposure of cells to heat-labile sonic extract for periods as brief as 10 min produced significant suppression of [3H]thymidine uptake 72 h later. I propose that alteration of epithelial barriers may be one pathogenic mechanism of this organism.
منابع مشابه
Antibacterial Effect of Iranian Green Tea and Black Tea against Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis and Prevotella intermedia
ABSTRACT Background and Objective: Anaerobic bacteria are the main cause of periodontitis. It has been shown that green tea and black tea have antibacterial effect. The aim of this study was to determine he antibacterial effect of Iranian green tea and black tea against Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis and Prevotella intermedia...
متن کاملIsolation and characterization of hemolytic genes from Actinobacillus actinomycetemcomitans.
Periodontopathic Actinobacillus actinomyceremcomitans produces hemolysin and other leukotoxins. In the present study, two distinct clones which lysed horse erythrocytes were isolated by screening genomic DNA libraries of A. actinomycetemcomitans ATCC 43718 on blood agar plates. DNA hybridization analysis indicates that there were two distinct hemolytic genes present. Sonicated extracts from bot...
متن کاملLong-term effect of heat shock protein 60 from Actinobacillus actinomycetemcomitans on epithelial cell viability and mitogen-activated protein kinases.
Our previous studies showed that bacterial heat shock protein 60 (hsp60) induces cultured epithelial cell proliferation within 24 h. Here we investigated the long-term effects of heat shock protein 60 isolated from Actinobacillus actinomycetemcomitans on skin keratinocyte (HaCaT cell line) viability and the cell signaling involved. Prolonged incubation in the presence of hsp60 increased the rat...
متن کاملSusceptibility of Streptococcus mutans and Actinobacillus actinomycetemcomitans to Bactericidal Activity of Human -Defensin 3 in Biological Fluids
Bactericidal activity of human -defensin 3 (hBD-3) against Streptococcus mutans and Actinobacillus actinomycetemcomitans was inhibited in a dose-dependent manner by the presence of saliva and/or serum. Increasing the concentration of hBD-3 partially overcame this inhibition. A fast bactericidal effect was observed against both bacterial strains, suggesting a potential therapeutic use for hBD-3 ...
متن کاملPurification and biochemical properties of a bacteriocin from Actinobacillus actinomycetemcomitans.
Extracts of certain strains of Actinobacillus actinomycetemcomitans are inhibitory to strains of Streptococcus sanguis such as S. sanguis ATCC 10556. The isolation of a protein from an A. actinomycetemcomitans sonic extract which copurified with the inhibitory activity was accomplished by preparative isoelectric focusing, Sephadex G-100 gel filtration chromatography, and preparative polyacrylam...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Infection and immunity
دوره 42 3 شماره
صفحات -
تاریخ انتشار 1983